Physiological variation among Tricholoma matsutake isolates generated from basidiospores obtained from one basidioma |
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Authors: | Akiyoshi Yamada Norio Hayakawa Chika Saito Yuka Horimai Hiroki Misawa Takashi Yamanaka Masaki Fukuda |
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Institution: | 1. Department of Agricultural and Life Science, Graduate School of Science and Technology, Shinshu University 8304, Minami-minowa, Nagano, 399-4598, Japan;2. Division of Terrestrial Ecosystem, Institute of Mountain Science, Shinshu University, 8304, Minami-minowa, Nagano, 399-4598, Japan;3. Department of Fungal Symbiotic Science & Resource Utilization Technology, Research Center for Fungal and Microbial Dynamism, Shinshu University, 8304, Minami-minowa, Nagano, 399-4598, Japan;4. Forestry and Forest Products Research Institute, Matsunosato 1, Tsukuba, Ibaraki, 305-8687, Japan |
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Abstract: | Matsutake (Tricholoma matsutake) is a commercially valuable edible ectomycorrhizal mushroom. The physiological traits of T. matsutake have been previously assessed using mycelial isolates isolated from basidiomata; however, few studies have focused on basidiospores. Here, we report that sibling T. matsutake isolates generated from basidiospores on a single basidioma show distinct physiological variation. We first established 145 isolates of T. matsutake on modified Norkrans' C (MNC) agar medium and found that their radial growth varied significantly. The mycelial biomasses of nine isolates with different growth rates were reduced on low-carbon and low-nitrogen MNC media. However, the colony diam of one isolate was significantly elevated on low-carbon medium, and the colony diam of two isolates were significantly elevated on low-nitrogen medium. In co-cultures of two or three isolates, commensal and amensal interactions were observed. The physiological variation induced by low carbon and nitrogen levels and the mycelial interactions between sibling isolates imply mechanisms for the genetic and functional characteristics of mycelia of T. matsutake. |
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Keywords: | Ectomycorrhizal symbiosis Genet interaction Soil microbial ecology Spore dispersal |
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