Optimization of culture conditions for glucose oxidase production by a Penicillium chrysogenum SRT 19 strain |
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Authors: | Ragini G Bodade Chandarahas N Khobragade Saiful Arfeen |
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Institution: | School of Life Sciences, Swami Ramanand Teerth Marathwada University, Nanded, Maharashtra, India |
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Abstract: | The enzyme glucose oxidase (GOD) has been used for a variety of biotechnological applications in food and pharmaceutical industries. In this study, the optimization of extracellular GOD production was carried out in a Penicillium chrysogenum SRT 19 strain isolated from contaminated and decaying cheese samples. Maximum GOD production was attained at pH 6 and 20°C in fermentation broth after 72 h of incubation. The effects of metal ions and sugars were screened for the induction of higher GOD production. The results revealed that glucose and lactose give the highest production of enzyme (0.670 and 0.552 U/mL, respectively) as compared with other sugars (sucrose, cellulose, mannitol and fructose). Out of the seven metal ions studied, CaCO3 (1.123 U/mL) and FeSO4 (0.822 U/mL) act as modulators, while MgSO4 (0.535 U/mL), CuSO4 (0.498 U/mL), HgCl2 (0.476 U/mL), ZnSO4 (0.457 U/mL) and BaSO4 (0.422 U/mL) yield lower production. The study therefore suggests that a strain of P. chrysogenum SRT 19 can be used as a new strain for GOD production. |
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Keywords: | Glucose oxidase Optimization Penicillium chrysogenum SRT 19 |
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