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Effect of light supply and carbon source on cell growth and cellular composition of a newly isolated microalga Chlorella vulgaris ESP‐31
Authors:Kuei‐Ling Yeh  Jo‐Shu Chang  Wen‐ming chen
Institution:1. Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan;2. Sustainable Environment Research Center, National Cheng Kung University, Tainan, Taiwan;3. Center for Bioscience and Biotechnology, National Cheng Kung University, Tainan, Taiwan;4. Department of Seafood Science, National Kaoshiung marine University, Kaoshiung, Taiwan
Abstract:Light supply is one of the most important factors affecting autotrophic growth of microalgae. This study investigated the effect of the type and light intensity of artificial light sources on the cell growth of an indigenous microalga Chlorella vulgaris ESP‐31 obtained from southern Taiwan. In addition, a dissolved inorganic carbon source (i.e. sodium bicarbonate) was used to improve the biomass production of strain ESP‐31. The results show that a new fluorescent light source (TL5) was effective in indoor cultivation of microalgae. Better overall productivity of 0.029 g dry cell weight/L‐d was obtained when using TL5 lamps as the light source with a light intensity of 9 W/m2. A carbon source (sodium bicarbonate) concentration of 1000 mg/L was found to be optimal for the growth of strain ESP‐31 in terms of both biomass production and carbon source utilization. Under the optimal growth conditions, the resulting microalgal biomass consisted of 25–30% protein, 6–10% carbohydrate, and 30–40% lipid.
Keywords:Cellular composition  Chlorella vulgaris  Light source  Microalgae  Sodium bicarbonate
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