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UCP4C mediates uncoupled respiration in larvae of Drosophila melanogaster |
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| Authors: | Caterina Da‐Ré Cristiano De Pittà Mauro A Zordan Giordano Teza Fabrizio Nestola Massimo Zeviani Rodolfo Costa Paolo Bernardi |
| Institution: | 1. Department of Biology, University of Padova, Padova, Italy;2. Department of Geosciences, University of Padova, Padova, Italy;3. MRC Mitocondrial Biology Unit, University of Cambridge, Cambridge, UK;4. Department of Biomedical Sciences, Consiglio Nazionale delle Ricerche Institute of Neuroscience, University of Padova, Padova, Italy |
| Abstract: | Larvae of Drosophila melanogaster reared at 23°C and switched to 14°C for 1 h are 0.5°C warmer than the surrounding medium. In keeping with dissipation of energy, respiration of Drosophila melanogaster larvae cannot be decreased by the F‐ATPase inhibitor oligomycin or stimulated by protonophore. Silencing of Ucp4C conferred sensitivity of respiration to oligomycin and uncoupler, and prevented larva‐to‐adult progression at 15°C but not 23°C. Uncoupled respiration of larval mitochondria required palmitate, was dependent on Ucp4C and was inhibited by guanosine diphosphate. UCP4C is required for development through the prepupal stages at low temperatures and may be an uncoupling protein. |
| Keywords: | Drosophila melanogaster energy dissipation mitochondria respiration uncoupling proteins |