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Initial phases of microbial biofilm formation on opaque,innovative anti‐adhesive surfaces using a modular microfluidic system
Authors:Katrin Wagner  Sandra Friedrich  Carolin Stang  Thomas Bley  Niels Schilling  Matthias Bieda  Andrés Lasagni  Elke Boschke
Institution:1. Institute of Food Technology and Bioprocess Engineering, Technische Universit?t Dresden, Dresden, Germany;2. Fraunhofer Institute for Material and Beam Technology IWS, Dresden, Germany
Abstract:Biofilms can cause numerous problems, hence it is important to understand their formation on surfaces in order to develop resistant materials and avoidance strategies. Therefore, information is required regarding adhesion processes on surfaces generally and innovative anti‐adhesive coatings in particular. Our flow cell system allows biofilms to be monitored in continuous flow conditions, without removing material for postflow imaging. The shown laminar flow ensures the maintenance of highly controlled conditions for biofilm growth. However, carried simulations of the oxygen demands of Escherichia coli cultivated as biofilms under the chosen regime indicate that conditions may become anaerobic, at least at the outlet of the flow cell, after a certain period of time. We report data on the biofouling tendencies on coatings generated with the help of direct laser interference patterning on stainless steel surfaces. Data were estimated from images acquired by fluorescence microscopy. Differences between patterned and unpatterned surfaces were not found, which is in accordance with the attachment point theory. Nevertheless, it is particularly important to elucidate in future studies the behavior of microorganisms during their attachment and the effects of variables of potentially sensitive surfaces (such as hydrophobicity, nanotopography, and charge) on their adhesion.
Keywords:Biofilm  Direct laser interference patterning  Flow chamber  Fluorescence microscopy  Nontransparent surfaces
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