Analysis,characterization and diagnostic use of circulating filarial antigen in bancroftian filariasis |
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Authors: | K A Parkhe M V R Reddy K Cheirmaraj P Ramaprasad B C Harinath |
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Institution: | (1) Department of Biochemistry, Mahatma Gandhi Institute of Medical Sciences, 442 102 Sevagram, Wardha, India |
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Abstract: | Sodium dodecyl sulphate-polyacrylamide gel electrophoresis of circulating filarial antigen fraction-2 isolated from plasma
of microfilaraemic patients withWuchereria bancrofti infection has shown 21 bands with molecular weights ranging from 12 to 120 kDa. The gel (12 cm) was sliced at an interval
of one cm and the eluates of all the gel slicesviz., CFA2-1 to CFA2-12 showed the presence of filarial antigen by sandwich enzyme-linked immunosorbent assay. The low molecular
weight circulating filarial antigen fractions were found to share a common epitope withWuchereria bancrofti microfilariae excretory-secretory antigen and urinary filarial antigen. The 3 antigen fractions CFA2-1, CFA2-9 and CFA2-12
showed higher sensitivity in detecting filarial immunoglobulin M antibodies than immunoglobulin G antibodies. However CFA2-9
fraction was found useful in serological differentiation of microfilaraemics from those with disease manifestations when filarial
immunoglobulin G antibodies were detected. The antigenic epitope of CFA2-1 appears to be a carbohydrate, whereas CFA2-9 appears
to be protein in nature. |
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Keywords: | Enzyme linked immunosorbent assay filarial serum immunoglobulin G circulating filarial antigen sodium dodecyl sulphate Polyacrylamide gel electrophoresis |
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