Iron uptake byDesulfovibrio vulgaris outer membrane components in artificial vesicles |
| |
| Authors: | G Bradley Dr C C Gaylarde |
| |
| Institution: | (1) Department of Biological Sciences, City of London Polytechnic, Old Castle Street, E1 7NT London, England;(2) Present address: Department of Biological Sciences, Plymouth Polytechnic, Drake Circus, PL4 8AA Plymouth, Devon, UK |
| |
| Abstract: | Desulfovibrio vulgaris lipopolysaccharide and outer membrane proteins (OMPs) were incorporated into vesicles ofD. vulgaris phospholipid and studied for 55Fe]binding activity. Both lipopolysaccharide and an extract of two major OMPs caused large increases in55Fe uptake over control (phospholipid only) vesicles. CommercialSalmonella typhimurium lipopolysaccharide gave a similar result, but the effect was inhibited by calcium ions; this was not the case forDesulfovibrio. The lipid A portion ofS. typhimurium lipopolysaccharide had a high iron-binding ability, whereasDesulfovibrio lipid A iron binding was little different from control values;D. vulgaris lipopolysaccharide thus has a specific iron-binding site within its polysaccharide side chain. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
