New insights into the Enterococcus faecalis CroRS two-component system obtained using a differential-display random arbitrarily primed PCR approach |
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| Authors: | Le Breton Yoann Muller Cécile Auffray Yanick Rincé Alain |
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| Affiliation: | Laboratoire Microbiologie de l'Environnement, EA 956, USC INRA 2017, IRBA, Université de Caen, France. ylebreton@umaryland.edu |
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| Abstract: | Using a modified random arbitrarily primed PCR approach, the operon encoding the Enterococcus faecalis JH2-2 CroRS two-component regulatory system was shown to be repressed during stationary phase, and a CroRS-regulated operon (glnQHMP) was identified. Gel retardation assays showed that the CroR regulator binds specifically to the glnQHMP promoter. |
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