Regulated multicistronic expression technology for mammalian metabolic engineering |
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Authors: | Martin Fussenegger Samuel Moser James E Bailey |
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Institution: | (1) Swiss Federal Institute of Technology, ETH Zurich, Institute of Biotechnology, CH-8093 Zurich, Switzerland |
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Abstract: | Contemporary basic research is rapidly revealing increasingly complex molecular regulatory networks which are often interconnected
via key signal integrators. These connections among regulatory and catalytic networks often frustrate bioengineers as promising
metabolic engineering strategies are bypassed by compensatory metabolic responses or cause unexpected, undesired outcomes
such as apoptosis, product protein degradation or inappropriate post- translational modification. Therefore, for metabolic
engineering to achieve greater success in mammalian cell culture processes and to become important for future applications
such as gene therapy and tissue engineering, this technology must be enhanced to allow simultaneous, in cases conditional,
reshaping of metabolic pathways to access difficult-to-attain cell states. Recent advances in this new territory of multigene
metabolic engineering are intimately linked to the development of multicistronic expression technology which allows the simultaneous,
and in some cases, regulated expression of several genes in mammalian cells. Here we review recent achievements in multicistronic
expression technology in view of multigene metabolic engineering.
This revised version was published online in August 2006 with corrections to the Cover Date. |
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Keywords: | autoregulation cell-cycle engineering eukaryotic operon IRES multigene engineering picornavirus pTRIDENT regulated expression |
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