STUDIES ON PROTEIN SYNTHESIS IN TOMATO COTYLEDONS AND LEAVES. I. PROTEIN SYNTHESIS AND TURNOVER IN INTACT COTYLEDONS AND LEAVES |
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Authors: | HALL, T.C. COCKING, E.C. |
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Affiliation: | Department of Botany, University of Nottingham Nottingham, England |
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Abstract: | - Details of aseptic culture of virus-free tomato seedlings usedin comparative in vivo and in vitro studies on protein synthesisare described.
- Developmental changes in the levels of DNA,RNA, protein andchlorophyll content of seedling cotyledonsand leaves were recorded,and are related to protein synthesis.
- Incorporation of isotopically labeled carbon into proteinwasfollowed both by photosynthetic uptake of 14CO2 and by theuptakeof 14C-amino acids through the roots.
- A marked stimulationby light of 14C uptake was observed, andthe higher rate of14C incorporation from 14CO2 than from 14C-aminoacids intothe protein fraction is discussed in relation tothe pathwaysof protein synthesis in tomato leaves, and alsowith regardto protein turnover.
1Present address: Dept. of Horticultural Science, Universityof Wisconsin, U.S.A. |
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