Identification of lactoferrin as an essential growth factor for human lymphocytic cell lines in serum-free medium |
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Authors: | Shuichi Hashizume Kazuhiko Kuroda Hiroki Murakami |
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Institution: | 1. Morinaga Institute of Biological Science, Shimosueyoshi 2-1-1, Tsurumi-ku, Yokohama 230 Japan;2. Department of Food Science and Technology, Kyushu University, Hakozaki 6-10-1, Higashi-ku, Fukuoka 812 Japan |
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Abstract: | A serum-free medium supplemented with growth factor(s) was devised to grow human lymphocytic cell lines. The medium was developed using human lymphocytic cell line, Bri 7 cells. In the process of constructing the medium, human lactoferrin was found to be an essential growth factor for the cell line. Human lactoferrin has higher growth stimulatory activity than human transferrin, and was sensitive to heat. Long-term cultivation of the cells was achieved in the defined medium supplemented with human lactoferrin only. The defined medium specifically supported the growth of various other human B- and T-lymphocytic cell lines but not the growth of various mouse lymphocytic cell lines. In lactoferrin-supplemented medium, the growth of some human cell lines were further stimulated by the addition of a combination of insulin, ethanolamine and selenium, or another combination of 2-mercaptoethanol and the above three factors. Bovine lactoferrin could be substituted for human lactoferrin. |
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Keywords: | Lactoferrin Serum-free medium Growth factor (Human lymphocyte) |
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