Murine cytotoxic T-cell response to alphavirus is associated mainly withH- 2D k

A secondary in vitro response to alphaviruses Bebaru, Sindbis, and Semliki Forest is described. Optimum response appears at day 5–6 of culture. The cells responsible for lytic activity are nonadherent, -positive, Ig^–, and mainly Ly-2.1 positive. Out of five haplotypes tested (H- 2 ^d ,H- 2 ^b ,H- 2 ^s ,H- 2 ^q , andH- 2 ^k ) onlyH- 2 ^k was a responder. Genetic mapping of the response located it solely in theD region of theH- 2 complex. The other four haplotypes responded with a high antiself activity after a second stimulation with viruses. This antiself response also maps in theD region of theH- 2 complex. No complementation was observed in F₁ hybrids between responder and nonresponder strains.