Stress-induced down-regulation of tumor-associated NADH oxidase during apoptosis in transformed cells |
| |
Authors: | Mao Liang-Chi Wang Hsi-Ming Lin You-Yu Chang Te-Kau Hsin Yi-Hong Chueh Pin Ju |
| |
Affiliation: | Institute of Biomedical Sciences, National Chung Hsing University, Taichung 40227, Taiwan, ROC. |
| |
Abstract: | Tumor-associated NADH oxidase (tNOX) is a growth-related protein expressed in transformed cells. tNOX knockdown using RNA interference leads to a significant reduction in HeLa cell proliferation and migration, indicating an important role for tNOX in growth regulation and the cancer phenotype. Here, we show that tNOX is down-regulated during apoptosis in HCT116 cells. Treatment with diverse stresses induced a dose- and time-dependent decrease in tNOX expression that was concurrent with apoptosis. Moreover, shRNA-mediated tNOX knockdown rendered cells susceptible to apoptosis, whereas re-expression of tNOX partially recovered cell proliferation. Our results indicate that tNOX is suppressed during apoptosis and demonstrate that tNOX down-regulation sensitizes cells to stress-induced growth reduction, suggesting that tNOX is required for transformed cell growth. |
| |
Keywords: | |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|