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Pseudomonas linyingensis sp. nov.: A Novel Bacterium Isolated from Wheat Soil Subjected to Long-term Herbicides Application
Authors:Wei-Hong He  Ya-Nan Wang  Xun Du  Yang Zhou  Bin Jia  Jiang Bian  Shuang-Jiang Liu  Guo-Can Chen
Institution:1. Henan Key Laboratory of Microbial Engineering, Institute of Biology, Henan Academy of Sciences, Zhengzhou, 450008, China
2. State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China
3. Department of Oral Biology, State University of New York at Buffalo, Buffalo, NY, 14214, USA
Abstract:A strain of genus Pseudomonas, LYBRD3-7T was isolated from long-term sulfonylurea herbicides applied wheat-field soil in Linying located in Henan province of China. This strain is a strictly aerobic and Gram-negative short rod-shaped bacterium with single flagellum. Phylogenetic evaluation based on 16S rRNA gene sequence analysis placed this isolate as a member of Pseudomonas, and most closely to Pseudomonas tuomuerensis CGMCC 1.1365T (97.1?%) and P. alcaligenes IAM12411T (97.1?%). Morphological characters and chemotaxonomic data confirmed the affiliation of strain LYBRD3-7T to the genus Pseudomonas. The results of phylogenetic analysis, physiological and biochemical studies, and DNA–DNA hybridization allowed the differentiation of genotype and phenotype between strain LYBRD3-7T and the phylogenetic closest species with valid names. The name proposed for the new species is Pseudomonas linyingensis sp. nov. The type strain is LYBRD3-7T (=CGMCC 1.10701T? =LMG 25967T).
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