农药残留检测关键用酶固定化研究进展

为保障消费者食用安全,迫切需要研发农产品和食品中的农药残留快速检测技术.酶抑制法检测是目前农药残留快速检测技术中的主要研究方向之一,而酶的固定化是用基于酶抑制法原理对农药残留检测研究中的重要步骤.通过物理或化学的方法高效地将酶固定于载体上,同时保持酶的催化活性是开发各类基于酶抑制法检测农药残留传感器的关键.本文将从固定...

Research Progress in the Immobilization of Key Enzymes for Pesticides Residue Detection

In order to detect pesticide residues in agro-products and foods to ensure consumers’ food safety,some fast detection techniques need to be developed urgently.Enzyme inhibition method is one of current main research directions in the rapid detection techniques of pesticide residues.While the immobilized enzyme based on the principle of enzyme inhibition method is an important step in the research of detecting pesticide residue.Applying physical or chemical methods to efficiently fix enzyme on the carrier without losing catalytic activity is the key step to develop all kinds of sensor based on enzyme inhibition method to detect pesticide residues.In this paper,the immobilized enzyme methods,carriers and the practical applications of immobilized enzyme products in pesticide residue detection are reviewed,so as to provide some experiences and references for the applications of immobilized enzyme in pesticide residue detection.