| 环境DNA宏条形码在生物多样性研究与监测中的应用 |
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| 引用本文： | 康子清,张银龙,吴永波,谢冬,薛建辉,华建峰.环境DNA宏条形码在生物多样性研究与监测中的应用[J].生物技术通报,2022(1):299-310. |
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| 作者姓名： | 康子清 张银龙 吴永波 谢冬 薛建辉 华建峰 |
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| 作者单位： | 南京林业大学生物与环境学院;南京林业大学江苏省南方现代林业协同创新中心;中国科学院江苏省植物研究所（南京中山植物园） |
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| 基金项目： | 江苏省农业科技自主创新资金项目[CX(18)2026];江苏省高校优势学科建设工程项目(PAPD)。 |
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| 摘 要： | 环境DNA宏条形码(eDNA metabarcoding)技术通过提取水体、土壤、空气中的环境DNA,使用引物PCR扩增与高通量测序,进行物种鉴定与生物多样性评估.作为一种新的监测技术,相比于传统监测技术更加快捷、准确以及对自然环境的破坏小,因此在一定程度上改变了我们调查地球生物多样性的方式.本文综述了环境DNA宏条形...
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| 关 键 词： | 环境DNA宏条形码 生物多样性 生物监测 |
Application of Environmental DNA Metabarcoding in Biodiversity Research and Monitoring |
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| KANG Zi-qing,ZHANG Yin-long,WU Yong-bo,XIE Dong,XUE Jian-hui,HUA Jian-feng.Application of Environmental DNA Metabarcoding in Biodiversity Research and Monitoring[J].Biotechnology Bulletin,2022(1):299-310. |
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| Authors: | KANG Zi-qing ZHANG Yin-long WU Yong-bo XIE Dong XUE Jian-hui HUA Jian-feng |
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| Institution: | (College of Biology and the Environment,Nanjing Forestry University,Nanjing 210037;Collaborative Innovation Center of Sustainable Forestry in Southern China of Jiangsu Province,Nanjing 210037;Institute of Botany,Jiangsu Province and Chinese Academy of Sciences,Nanjing 210014) |
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| Abstract: | Environmental DNA metabarcoding(eDNA)is used to identify species and assess biodiversity by extracting environmental DNA from water,soil and air and using primers for PCR amplification and high-throughput sequencing.As a new monitoring technology,it is faster,more accurate and less destructive to the natural environment while compared with traditional monitoring technology.Therefore,it has changed the way we investigate the biodiversity of the earth to some extent.In this paper,the development and operation process of environmental DNA metabarcoding technology are reviewed.The application status in water,soil and air environment is summarized,and the unique indirect biodiversity monitoring methods are summarized.Concurrently,the advantages and existing problems are discussed in order to provide new ideas and inspirations for the follow-up research and monitoring of biodiversity. |
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| Keywords: | eDNA metabarcoding biodiversity biomonitoring |
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