| 单分子荧光检测技术的发展及其在植物生物学研究中的应用 |
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| 引用本文： | 张原,张雪萍,张月倩,李晓娟. 单分子荧光检测技术的发展及其在植物生物学研究中的应用[J]. 生物技术通报, 2022, 0(1): 33-43 |
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| 作者姓名： | 张原 张雪萍 张月倩 李晓娟 |
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| 作者单位： | 北京林业大学生物科学与技术学院林木育种国家工程实验室北京林业大学林木分子设计育种高精尖创新中心 |
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| 基金项目： | 国家自然科学基金项目(91954202,31871349);北京林业大学科技创新计划项目(2019JQ03003)。 |
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| 摘 要： | 单分子荧光检测技术是利用荧光基团对目的分子标记后,在单分子水平成像并追踪分子的构象变化、动力学特征以及分子之间相互作用的研究方法.相较于传统分子生物学和遗传学的研究手段,单分子检测技术可以对单个分子的动态和特性进行分析,特别是瞬时或偶发性的事件,从而更加深入地挖掘在群体测量中被掩盖的信息.该技术已广泛应用于动物细胞生物...
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| 关 键 词： | 单分子 荧光成像 光谱检测 植物生物学 |
Advances of Single-molecule Fluorescence Detection Techniques and Applications in Plant Biology |
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| ZHANG Yuan,ZHANG Xue-ping,ZHANG Yue-qian,LI Xiao-juan. Advances of Single-molecule Fluorescence Detection Techniques and Applications in Plant Biology[J]. Biotechnology Bulletin, 2022, 0(1): 33-43 |
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| Authors: | ZHANG Yuan ZHANG Xue-ping ZHANG Yue-qian LI Xiao-juan |
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| Affiliation: | (College of Biological Sciences and Technology,National Engineering Laboratory for Tree Breeding,Beijing Advanced Innovation Center for Tree Breeding by Molecular Design,Beijing Forestry University,Beijing 100083) |
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| Abstract: | Single-molecule fluorescence detection techniques(SMD)are used to image and track the conformational changes,dynamics and interactions of fluorescently labeled molecules at single-molecule level.Compared with the traditional molecular and genetic approaches,SMD can analyze the dynamical characteristics of individual molecules,especially instantaneous and occasionally events that may be hidden in massive average measurements.Therefore,SMD has been widely used in animal researches,which made numerous important contributions to molecular mechanisms in biology researches.However,the application of SMD in plants remains to be further developed.In this review,we introduced the most recently advances of SMD techniques.Then we summarized its application cases in plant biology,as well as the application prospect of SMD in plant biology researches in the near future. |
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| Keywords: | single-molecule fluorescence imaging spectroscopy detection plant biology |
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