蒺藜苜蓿MtSAG113基因的转化及表达特征分析

Senescence Associated Gene 113(SAG113)基因属于PP2Cc超家族,该基因的研究主要集中在植物衰老领域.为分析蒺藜苜蓿(Medicago truncatula)MtSAG113基因的表达特征,探究MtSAG113基因的功能.该基因从蒺藜苜蓿中克隆得到,以烟草(Nicotiana tab...

Transformation and Expression Pattern Analysis of Gene MtSAG113 from Medicago truncatula

Senescence Associated Gene 113(SAG113)genes belong to PP2Cc super family and they are involved in plant senescence.This work aims to analyze the expression characteristics of the gene MtSAG113 of Medicago truncatula and to explore the function of the MtSAG113.This gene was cloned from M.truncatula.Using Nicotiana tabacum L.and M.truncatula R108 as experimental materials,the Agrobacterium-mediated leaf disc method was used to transform tobacco to obtain transgenic plants with resistance.The expression of MtSAG113 from M.truncatula was detected under induction of stress and exogenous hormones.The results showed that 5 transgenic plants successfully expressed in tobacco were obtained by PCR detection and the transgenic plants presented the phenotype of shortness and premature leaf senescence compared with the wild ones.The expressions of MtSAG113 in the senescence leaves were much higher than those in non-senescence leaves.In untreated leaves,the expression of this gene gradually increased with time and the gene was significantly up-regulated expressed under drought stresses.The expressions were significantly up-regulated under salt stresses and reached the peak when suffered for 12 h.The gene was particularly sensitive to 6-BA induction,and the expression increased significantly with time increasing,reached a peak at 4 h,which was about 1 000 times higher than those untreated.Treated with ABA and IAA,MtSAG113 exhibited a similar expression pattern,the expression increased from 4 h,reached a peak at 12 h.The subcellular localization results demonstrated that the gene was located in the nucleus.Above results indicate that MtSAG113 may play a certain role in senescence regulation in M.truncatula,and high salinity,drought and exogenous hormones regulate the expression of MtSAG113,which provides a basis for further function analysis of MtSAG113.