Optimization of APE1547-catalyzed enantioselective transesterification of (R/S)-2-methyl-1-butanol in an ionic liquid |
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| Authors: | Rui Tian Chun-Hua Yang Xiao-Fei Wei Er-Na Xun Ren Wang Shu-Gui Cao Zhi Wang Lei Wang |
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| Institution: | (1) Institute of Life Technologies, University of Applied Sciences Western Switzerland, HES-SO Valais, Route du Rawyl 64, 1950 Sion 2, Switzerland;(2) Institute Systems Engineering, University of Applied Sciences Western Switzerland, HES-SO Valais, Route du Rawyl 47, 1950 Sion 2, Switzerland |
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| Abstract: | Aeropyrum pernix esterase (APE1547) was successfully used to catalyze the enantioselective transesterification of (R/S)-2-methyl-1-butanol
in an ionic liquid (IL). Effects of various reaction conditions on the synthetic activity of the enzyme as well as enantioselectivity,
including the type of IL, acyl donor, temperature, water activity, and substrate molar ratio were inverstigated. APE1547 showed
good catalytic performance (activity > 0.8 μmol/min/mg, E > 25), and the enzyme-IL mixture was recycled five times with only
a slight decrease in catalytic performance. |
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