Molecular characterization reveals involvement of altered el tor biotype <Emphasis Type="Italic">Vibrio cholerae</Emphasis> O1 strains in cholera outbreak at Hyderabad,India |
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Authors: | Ajay Kumar Goel Meenu Jain Pramod Kumar Pennagaram Sarguna Meera Bai Neha Ghosh Natrajan Gopalan |
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Institution: | (1) Biotechnology Division, Defence Research and Development Establishment, Gwalior, 474 002, India;(2) Department of Civil and Environmental Engineering, University of California, Irvine, CA 92697, USA |
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Abstract: | Thirty-four Vibrio cholerae isolates collected from a cholera outbreak in Hyderabad, South India were found to belong to serogroup Ol biotype El Tor
serotype Ogawa. The genotype of all the isolates was confirmed by PCR assays. All the isolates were found PCR positive for
ctxAB, ompW, rflOl, rtxC, and tcpA genes. All the isolates but one harboured rstR
El Tor
allele. However, one isolate carried both rstR
EL Tor
as well as rstR
Classical
alleles. Cholera toxin (ctxB) genotyping of the isolates confirmed the presence of altered cholera toxin B of classical biotype in all the isolates. All
the isolates except VCH35 harboured an RS1-CTX prophage array on the large chromosome. The isolate VCH35 contained a tandem
repeat of classical CTX prophage on the small chromosome. The clonal relationship among the V. cholerae isolates as carried out by enterobacterial repetitive intergenic consensus sequences PCR, BOX PCR and randomly amplified
polymorphic DNA, uniformly showed a genetic relationship among the outbreak isolates. The results of this study suggest that
altered El Tor biotype V. cholerae with the classical cholera toxin gene are involved in cholera outbreaks in India. |
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Keywords: | |
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