Distinct fucosylation of M cells and epithelial cells by Fut1 and Fut2, respectively, in response to intestinal environmental stress |
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| Authors: | Terahara Kazutaka Nochi Tomonori Yoshida Masato Takahashi Yuko Goto Yoshiyuki Hatai Hirotsugu Kurokawa Shiho Jang Myoung Ho Kweon Mi-Na Domino Steven E Hiroi Takachika Yuki Yoshikazu Tsunetsugu-Yokota Yasuko Kobayashi Kazuo Kiyono Hiroshi |
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| Affiliation: | aDepartment of Immunology, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan;bDivision of Mucosal Immunology, Department of Microbiology and Immunology, The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan;cLaboratory of Gastrointestinal Immunology, World Premier International Immunology Frontier Research Center, Osaka University, 3-1 Yamada-oka, Suita, Osaka 565-0871, Japan;dMucosal Immunology Section, International Vaccine Institute, Seoul 151-818, Republic of Korea;eDepartment of Obstetrics and Gynecology, The University of Michigan Medical School, 6428 Medical Science Bldg I, 1150 West Medical Center Drive, Ann Arbor, MI 48109-5617, USA;fDepartment of Allergy and Immunology, The Tokyo Metropolitan Institute of Medical Science, 2-1-6 Kamikitazawa, Setagaya-ku, Tokyo 156-8506, Japan |
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| Abstract: | The intestinal epithelium contains columnar epithelial cells (ECs) and M cells, and fucosylation of the apical surface of ECs and M cells is involved in distinguishing the two populations and in their response to commensal flora and environmental stress. Here, we show that fucosylated ECs (F-ECs) were induced in the mouse small intestine by the pro-inflammatory agents dextran sodium sulfate and indomethacin, in addition to an enteropathogen derived cholera toxin. Although F-ECs showed specificity for the M cell-markers, lectin Ulex europaeus agglutinin-1 and our monoclonal antibody NKM 16-2-4, these cells also retained EC-phenotypes including an affinity for the EC-marker lectin wheat germ agglutinin. Interestingly, fucosylation of Peyer’s patch M cells and F-ECs was distinctly regulated by α(1,2)fucosyltransferase Fut1 and Fut2, respectively. These results indicate that Fut2-mediated F-ECs share M cell-related fucosylated molecules but maintain distinctive EC characteristics, Fut1 is, therefore, a reliable marker for M cells. |
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| Keywords: | Epithelial cell Fucosyltransferase Intestine M cell Peyer&rsquo s patch |
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