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The incorporation of radioactive uridine into the hepatitis B antigen of a chimpanzee. |
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| Authors: | W Jó?wiak J Desmyter A O'Connell J Mortelmans I Millman |
| Abstract: | Radioactive (3H) uridine was incorporated into RNA isolated from the HBsAg of a chimpanzee carrier. HBsAg was purified by precipitation as an immune complex with the IgG fraction of chimpanzee anti-HBs. RNA was extracted from the washed complex with buffered phenol precipitated with alcohol and four nucleotides were identified by thin layer chromatography after alkali degradation. |
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