| 合成乙型肝炎表面抗原肽段的结构与抗原性 |
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| 引用本文： | 胡培声,刘志平,施溥涛,黄惟德.合成乙型肝炎表面抗原肽段的结构与抗原性[J].中国生物化学与分子生物学报,1985,1(4):79-86. |
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| 作者姓名： | 胡培声 刘志平 施溥涛 黄惟德 |
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| 作者单位： | 中国科学院上海生物化学究所
(胡培声,刘志平,施溥涛),中国科学院上海生物化学究所(黄惟德) |
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| 摘 要： | 本文对合成的乙型肝炎表面抗原肽段的结构与抗原性进行了研究。通过对三种亚型共9个合成肽段的抗原性测定和结构分析,我们证实了在乙型肝炎表面抗原(HBsAg)氨基酸顺序的122-137区域存在着共同决定簇“a”,且半胱氨酸残基对抗原性有很大的影响。合成的16肽P_(122-137)的两个半胱氨酸残基用叔丁基保护时,其抗原性几乎检测不到,一旦去掉保护基并氧化成分子内S—S键后,抗原性明显增加。比较各种亚型肽段的抗原性测定结果,我们发现亚型决定簇d(或y)的位置可能在122—132之间。另外我们发现P_(adw)122—132的抗原性要比P_(adr)122-137的抗原性强,结构分析结果表明adw型中的Asn_(132)可能对此有较大的贡献。
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| 关 键 词： | 乙型肝炎表面抗原肽段合成 乙型肝炎表面抗原结构合成疫苗 结构-抗原性 肽段抗原性 |
| 收稿时间： | 1985-08-20 |
Structure and Antigenicity of Synthetic HBsAg Peptides |
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| Hu Pei-sheng,Liu Zhi-ping,Shi Fu-tao,Huang Wei-tehshanghai Instituse of Biochemistry,Academia Sinica.Structure and Antigenicity of Synthetic HBsAg Peptides[J].Chinese Journal of Biochemistry and Molecular Biology,1985,1(4):79-86. |
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| Authors: | Hu Pei-sheng Liu Zhi-ping Shi Fu-tao Huang Wei-tehshanghai Instituse of Biochemistry Academia Sinica |
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| Abstract: | A series of research on structure and antigenicity of 9 synthetic peptides of hepatities B surface antigen (HBsAg) with different subtype were carried out.The results indicated that the peptide sequence 122-137 contains an essential part of the "av"determinant of HBsAg.The antigenicity of P122-137 which contains two Cys was poor when the Cys was alkylated with t-Butyl group and greatly increaced as the t-Butyl was removed and the peptide was oxidized. In addition, we located the "d"antigenic site between residures 122 and 132. The contribution of Asn 132 to antigenicity of Padw122-132 was also discussed. |
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| Keywords: | Synthetic HBsAg peptides HBsAg structure Synthetic vaccine Structure-antigenicity Peptide antigenicity |
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