The identification of a periplasmic nitrate reductase in Paracoccus denitrificans |
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| Authors: | Heather J. Sears Stuart J. Ferguson David J. Richardson Stephen Spiro |
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| Affiliation: | School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, UK; Department of Biochemistry, University of Oxford, Oxford OX1 3QU, UK |
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| Abstract: | Abstract A nitrate reductase activity has been identified in periplasmic extracts of Paracoccus denitrificans . The enzyme is relatively insensitive to azide and does not reduce chlorate, features which distinguish it from the well-characterised membrane-associated nitrate reductase. The specific activity of the enzyme was higher in intact cells grown with butyrate rather than succinate as the sole source of carbon. |
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| Keywords: | Paracoccus denitrificans Aerobic denitrification Nitrate reductase Periplasm |
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