贯叶连翘对应激大鼠生长和脑单胺类神经递质含量的影响

为探讨贯叶连翘对慢性应激大鼠生长和脑单胺类神经递质的影响,用15只大鼠设置对照组、应激组和贯叶连翘组3组实验。应激组和贯叶连翘组均进行7天的应激刺激后,贯叶连翘组灌胃贯叶连翘10d。实验结束后,取3组大鼠的脑组织,用高效液相色谱法测定高香草酸(HVA)、去甲肾上腺素(NE)、多巴胺(DA)和5-羟色胺(5-HT)的含量。结果表明,应激组大鼠日增重明显低于对照组;而贯叶连翘组大鼠的日增重明显高于应激组。应激组大鼠海马、纹状体和前额叶中的HVA、NE、DA和5-HT与对照组间均无显著差异。贯叶连翘组大鼠纹状体中的DA含量明显高于应激组;而前额叶中的DA则明显低于应激组。因此,贯叶连翘对慢性应激引起的大鼠生长受抑有缓解作用,对其脑内单胺类神经递质有部分调节作用。

Effect of Hypericum perforatum on Growth and Brain Monoamine Neurotransmitters in Stressed Rats

This study aimed to investigate the effects of Hypericum perforatum on growth and brain monoamine neurotransmitters in chronic-stressed rats. Fifteen individuals were divided into three groups including control, stressed and treated with H. perforatum group. The rats in both stressed and the H. perforatum groups were stressed for 7 days, and the rats in the latter group were fed with H. perforatum for 10 days after stress. The brain tissues of the rats in all three groups were collected to analyse the content of homovanillic acid (HVA), norepinephrine (NE), dopamine (DA) and5-hydroxytryptamine (5-HT) after the experiment was finished. The indexes above were determined by the method of high pressure liquid chromatogram (HPLC). The results showed that the daily growth mass in the stressed group significantly decreased compared with the control group, while the ones of H. perforatum group were higher than those of the stressed group. The HVA levels of the Hippocampus,striatum and prefrontal lobe in the stressed group had no significant differences compared with the control group, though there was an increase tendency in the stressed group. The stress had no significant effect on the levels of NE, DA and 5-HT. The DA levels of striatum in the H. perforatum group were significantly higher than those in the stressed group, while DA levels of prefrontal lobe were significantly decreased compared with the stressed group. These results suggest that H. perforatum can alleviate the growth decrease caused by stress, and can partly regulate the levels of monoamine neurotransmitters in the brain of stressed rats.