Sporulation of Spheroplasts in Saccharomyces cerevisia

Spheroplasts of Saccharomyces cerevisiae sporulated in 0.1 Mpotassium acetate solution which contained 0.8 M sorbitol ormannitol as the osmotic stabilizer. The appearance of matureasci in both spheroplasts and intact cells was retarded by theaddition of the osmotic stabilizer. Sporulation was repressedmarkedly when 0.6 M KCl was used as the osmotic stabilizer inthe sporulation medium. The germination rate of the spores formedin spheroplasts was 97%. Tetrad analysis showed that meiosiswas normal during the sporulation of spheroplasts. (Received September 5, 1980; Accepted November 29, 1980)