A novel dodecadepsipeptide, cereulide, isolated from Bacillus cereus causes vacuole formation in HEp-2 cells |
| |
| Authors: | Norio Agata Masashi Mori Michio Ohta Sathorn Suwan Ikuko Ohtani Minoru Isobe |
| |
| Institution: | Nagoya City Public Health Research Institute, 1-11 Hagiyamacho, Mizuho, Nagoya 467, Japan; Department of Bacteriology, Nagoya University School of Medicine, 65 Tsurumaicho, Showa, Nagoya 466, Japan; Laboratory of Organic Chemistry, School of Agricultural Sciences, Nagoya University, Chikusa, Nagoya 464-01, Japan |
| |
| Abstract: | Abstract A HEp-2 cell-vacuolation factor was extracted and purified from the culture supernatant of a Bacillus cereus strain which caused emetic-syndrome food poisoning. The final preparation was chemically pure, and the toxin was named as cereulide. Mass spectrometry, NMR studies and chemical degradation revealed that the cereulide is a cyclic dodecadepsipeptide, (D-O-Leu-D-Ala- L-O-Val-L-Val)3, which is closely related to the potassium ionophore, valinomycin. |
| |
| Keywords: | Bacillus cereus Dodecadepsipeptide Cereulide Vacuolation Emetic toxin |
|
|
