Dimethyl suberimidate cross-linking of oligo(dT) to DNA-binding proteins |
| |
Authors: | Dodson M S |
| |
Institution: | Department of Biochemistry and Molecular Biophysics, University of Arizona, Tucson, Arizona 85721-0088, USA. |
| |
Abstract: | Dimethyl suberimidate is a bifunctional reagent that is used for cross-linking the protein components of oligomeric macromolecules. In this report, dimethyl suberimidate is shown to specifically cross-link oligo(dT) of varying lengths to the DNA-binding subunits of a multimeric helicase-primase encoded by herpes simplex virus type 1. This result indicates that dimethyl suberimidate and other imidoester cross-linking reagents may be useful for characterizing the interaction of oligo(dT) with proteins that bind single-stranded DNA. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|