Isolation and characterization of polymorphic microsatellite DNA markers in the rock bream (Oplegnathus fasciatus) |
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| Authors: | Tianjun Xu Changwei Shao Xiaolin Liao Xiangshan Ji Songlin Chen |
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| Institution: | (1) Key Laboratory for Sustainable Utilization of Marine Fisheries Resource, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Nanjing Road 106, Qingdao, 266071, China;(2) College of Aqua-life Science and Technology, Shanghai Fisheries University, Shanghai, 200090, China |
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| Abstract: | From a (GT)13-enriched genomic library of Oplegnathus fasciatus, 14 polymorphic microsatellite were isolated and characterized in a test population with alleles ranging from two to nine,
the observed and expected heterozygosities from 0.0000 to 1.0000, and from 0.1726 to 0.8507, respectively. Five loci deviated
from the Hardy–Weinberg equilibrium, and linkage disequilibrium between two loci was significant. Two loci were also polymorphic
in Pagrosomus major assessed for cross-species amplification. These polymorphic microsatellites will be useful for genetic diversity analysis
and molecule-assisted breeding for O. fasciatus.
Changwei Shao contributed equally. |
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| Keywords: | Oplegnathus fasciatus Enriched genomic library Microsatellites |
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