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Immunocytochemical demonstration of a humoral defence factor in blood cells (amoebocytes) of the pond snail,Lymnaea stagnalis
Authors:W. P. W. van der Knaap  L. H. Boerrigter-Barendsen  D. S. P. van den Hoeven  T. Sminia
Affiliation:(1) Biological Laboratory, Vrije Universiteit, Amsterdam, The Netherlands;(2) Department of Histology, Medical Faculty, Vrije Universiteit, Amsterdam, The Netherlands;(3) Biological Laboratory, Vrije Universiteit, DeBoelelaan 1087, 1007 MC Amsterdam, The Netherlands
Abstract:Summary Monolayers of blood cells (amoebocytes) and sections of connective tissue and of amoebocyte pellets of the freshwater pulmonate gastropod Lymnaea stagnalis were stained immunocytochemically with antisera to snail agglutinin/opsonin. The presence of this substance was demonstrated light microscopically both in the cytoplasm and on the cell membrane of amoebocytes. This suggests that amoebocytes synthesize agglutinin/opsonin, and bear it at their surface as receptors for foreign materials.
Keywords:Opsonin  Agglutinin  Amoebocytes  Immunocytochemistry  Lymnaea stagnalis
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