Cell proliferation in chicken intestinal epithelium occurs both in the crypt and along the villus |
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Authors: | Z Uni R Platin D Sklan |
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Institution: | (1) Faculty of Agriculture, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, Rehovot 76-100, Israel Tel.: +972-8-9481205, Fax:+972-8-9465763 e-mail: Uni@agri.huji.ac.il, IL |
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Abstract: | The location of cell proliferation and differentiation in chicken small intestinal epithelium was examined using immunostaining,
measurement of DNA synthesis and brush-border enzyme activities. Chicken enterocytes were removed sequentially from the villus
using a modification of the Weiser (1973) method. Alkaline phosphatase activity was relatively constant along the villus tip-crypt
axis but decreased in the crypt fractions, whereas sucrase and maltase activities showed higher activity in the upper half
of the villus and lower activity in the lower half of the villus and in the crypt. Immunostaining of proliferating cell nuclear
antigen indicated the presence of proliferating cells both in the crypt and along the villus, including some activity in the
upper portion; the crypt region exhibited a significantly higher number of proliferating cells. Labelled thymidine incorporation
into cell fractions after 2 h incubation exhibited a similar pattern of proliferation, with the most active region observed
in the crypt and proliferation activity decreasing along the villus. However, some activity was found in the upper half of
the villus. After 17 h incubation, cells from the middle region of the villi showed greater proliferation ability than the
2 h incubation. These results indicate that, unlike mammals, chicken enterocyte proliferation is not localized only in the
crypt region, and that the site of enterocyte differentiation is not precisely localized.
Accepted: 22 January 1998 |
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Keywords: | Chicken small intestine PCNA Cell proliferation |
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