In vitro production of radiolabeled red clover (<Emphasis Type="Italic">Trifolium pratense</Emphasis>) isoflavones |
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Authors: | Nancy J Engelmann Adam Reppert Gad Yousef Randy B Rogers Mary Ann Lila |
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Institution: | (1) Division of Nutritional Sciences, University of Illinois, 1201 S. Dorner Dr., Urbana, IL 61801, USA;(2) Department of Natural Resources and Environmental Sciences, University of Illinois, 1201 S. Dorner Dr., Urbana, IL 61801, USA |
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Abstract: | Red clover isoflavones are increasingly used in dietary supplements for their purported estrogenic effects. However, little
is known about their metabolism in animals due to a lack of commercially available isotopically labeled tracers. The goal
of this research was to establish red clover cell culturing methodology for 14C-biolabeling of isoflavones. When root, leaf, and petiole-derived suspension cultures were grown in darkness or light, dark-grown,
petiole-derived solution cultures produced the highest concentrations of the two major red clover isoflavones, formononetin
(0.67 mg/g FM inoculum) and biochanin A (0.13 mg/g FM inoculum). Varying levels and timing of copper chloride elicitor did
not significantly affect isoflavone accumulation. Approximately 38% of the 14C-sucrose dose accumulated in the cells. Eighteen percent of the initial labeled dose was detected in the isoflavone-rich
methanolic extract and of that, 22% accumulated in isoflavones. |
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Keywords: | Red clover Isoflavones Metabolic tracers Plant cell culture Copper chloride Radiolabeling Trifolium pratense |
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