| EDTA-induced Membrane Fluidization and Destabilization： Biophysical Studies on Artificial Lipid Membranes |
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| 作者姓名： | Prachayasittikul V Isarankura-Na-Ayudhya C Tantimongcolwat T Nantasenamat C Galla HJ |
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| 作者单位： | [1]Department of Clinical Microbiology, Faculty of Medical Technology, Mahidol University, Bangkok 10700, Thailand [2]Institute of Biochemistry, Westfalische Wilhelms Universitat, Muenster 48149, Germany |
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| 基金项目： | This work was supported by the grants from the Deutsche Forschungsgemeinschaft and the Bundesministerium fiir Wirtschaftliche Zusammenarbeit und Entwicklung （Federal Ministry for Economic Cooperation and Development） （No. GA233/19-1,2）, also from the Thailand Toray Science Foundation and the Thailand Governmental Budget of Mahidol University （No. 02012053-0003） |
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| 摘 要： | The molecular mechanism of ethylenediaminetetraacetic acid （EDTA）-induced membrane destabilization has been studied using a combination of four biophysical techniques on artificial lipid membranes. Data from Langmuir film balance and epifluorescence microscopy revealed the fluidization and expansion effect of EDTA on phase behavior of monolayers of either 1,2-dipalmitoyl-sn-glycero-3-phosphocholine （DPPC） or mixtures of DPPC and metal-chelating lipids, such as N^a,N^a-Biscarboxymethyl]-N^ε （dioctadecylamino）succinyl]-L-lysine or 1,2-dioleoyl-sn-glycero-3-N-（5-amino- 1 -carboxypentyl iminodiacetic acid） succinyl]. A plausible explanation could be drawn from the electrostatic interaction between negatively charged groups of EDTA and the positively charged choline head group of DPPC. Intercalation of EDTA into the lipid membrane induced membrane curvature as elucidated by atomic force microscopy. Growth in size and shape of the membrane protrusion was found to be time-dependent upon exposure to EDTA. Further loss of material from the lipid membrane surface was monitored in real time using a quartz crystal microbalance. This indicates membrane restabilization by exclusion of the protrusions from the surface. Loss of lipid components facilitates membrane instability, leading to membrane permeabilization and lysis.
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| 关 键 词： | 原子 显微镜 石英 结晶体 |
| 修稿时间： | 2007-03-22 |
EDTA-induced membrane fluidization and destabilization: biophysical studies on artificial lipid membranes |
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| Prachayasittikul V,Isarankura-Na-Ayudhya C,Tantimongcolwat T,Nantasenamat C,Galla HJ.EDTA-induced membrane fluidization and destabilization: biophysical studies on artificial lipid membranes[J].Acta Biochimica et Biophysica Sinica,2007,39(11):901-913. |
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| Authors: | Prachayasittikul Virapong Isarankura-Na-Ayudhya Chartchalerm Tantimongcolwat Tanawut Nantasenamat Chanin Galla Hans-Joachim |
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| Institution: | Department of Clinical Microbiology, Faculty of Medical Technology, Mahidol University, Bangkok 10700, Thailand;;Institute of Biochemistry, Westfälische Wilhelms Universität, Muenster 48149, Germany |
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| Abstract: | The molecular mechanism of ethylenediaminetetraacetic acid (EDTA)-induced membrane destabilization has been studied using a combination of four biophysical techniques on artificial lipid membranes. Data from Langmuir film balance and epifluorescence microscopy revealed the fluidization and expansion effect of EDTA on phase behavior of monolayers of either 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) or mixtures of DPPC and metal-chelating lipids, such as N(alpha),N(alpha)-Biscarboxymethyl]-N(epsilon)-(dioctadecylamino)succinyl]-L-lysine or 1,2-dioleoyl-sn-glycero-3-N-(5-amino-1-carboxypentyl iminodiacetic acid) succinyl]. A plausible explanation could be drawn from the electrostatic interaction between negatively charged groups of EDTA and the positively charged choline head group of DPPC. Intercalation of EDTA into the lipid membrane induced membrane curvature as elucidated by atomic force microscopy. Growth in size and shape of the membrane protrusion was found to be time-dependent upon exposure to EDTA. Further loss of material from the lipid membrane surface was monitored in real time using a quartz crystal microbalance. This indicates membrane restabilization by exclusion of the protrusions from the surface. Loss of lipid components facilitates membrane instability, leading to membrane permeabilization and lysis. |
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| Keywords: | ethylenediaminetetraacetic acid film balance atomic force microscopy quartz crystal microbalance membrane fluidization and destabilization |
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