Liposome-entrapped superoxide dismutase reduces ischemia/reperfusion ‘oxidative stress’ in gerbil brain |
| |
Authors: | Danica B Stanimirovic MD Marina Markovic Dejan V Micic Maria Spatz Bogomir B Mrsulja |
| |
Institution: | (1) Institute for Medical Research, Military Medical Academy, Belgrade, Yugoslavia;(2) School of Medicine, Institute of Biochemistry, Belgrade, Yugoslavia;(3) Stroke Branch, NINDS, National Institutes of Health, Bethesda, Maryland;(4) Cellular Neurobiology Group, Institute for Biological Sciences, National Research Council of Canada, Montreal Road, Bldg. M-54, K1A 0R6 Ottawa, Ontario, Canada |
| |
Abstract: | Bilateral common carotid artery occlusion (15 min.) followed by two hours of recirculation reduced mitochondrial superoxide dismutase (SOD) and glutathione reductase (GR) activities, and increased susceptibility of mitochondrial membranes to in vitro lipid peroxidation in brain regions (i.e., cortex, striatum and hippocampus) of Mongolian gerbil. Intraperitoneal bolus injection (2 mg/kg b.w.) of liposome-entrapped CuZn superoxide dismutase (l-SOD) increased the endogenous SOD activity in normal brain tissue and, when given at the end of ischemia, counteracted both the ischemic reduction of endogenous SOD and the increased peroxidation of mitochondrial membranes. 1-SOD treatment was ineffective in reducing brain swelling, suggesting that superoxide radicals are not a main participant in the process of (post)ischemic brain edema formation. |
| |
Keywords: | Brain ischemia reperfusion superoxide anion liposome-entrapped superoxide dismutase brain edema |
本文献已被 SpringerLink 等数据库收录! |
|