Generation of cloned transgenic cats expressing red fluorescence protein |
| |
Authors: | Yin Xi Jun Lee Hyo Sang Yu Xian Feng Choi Eugene Koo Bon Chul Kwon Mo Sun Lee Young S Cho Su Jin Jin Guang Zhen Kim Lyoung Hyo Shin Hyoung Doo Kim Teoan Kim Nam Hyung Kong Il Keun |
| |
Institution: | Department of Animal Science and Technology, Sunchon National University, Sunchon 540-742, South Korea. |
| |
Abstract: | A method for engineering and producing genetically modified cats is important for generating biomedical models of human diseases. Here we describe the use of somatic cell nuclear transfer to produce cloned transgenic cats that systemically express red fluorescent protein. Immature oocytes were collected from superovulating cat ovaries. Donor fibroblasts were obtained from an ear skin biopsy of a white male Turkish Angora cat, cultured for one to two passages, and subjected to transduction with a retrovirus vector designed to transfer and express the red fluorescent protein (RFP) gene. A total of 176 RFP cloned embryos were transferred into 11 surrogate mothers (mean = 16 +/- 7.5 per recipient). Three surrogate mothers were successfully impregnated (27.3%) and delivered two liveborn and one stillborn kitten at 65 to 66 days of gestation. Analysis of nine feline-specific microsatellite loci confirmed that the cloned cats were genetically identical to the donor cat. Presence of the RFP gene in the transgenic cat genome was confirmed by PCR and Southern blot analyses. Whole-body red fluorescence was detected 60 days after birth in the liveborn transgenic (TG) cat but not in the surrogate mother cat. Red fluorescence was detected in tissue samples, including hair, muscle, brain, heart, liver, kidney, spleen, bronchus, lung, stomach, intestine, tongue, and even excrement of the stillborn TG cat. These results suggest that this nuclear transfer procedure using genetically modified somatic cells could be useful for the efficient production of transgenic cats. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|