Determination of free sulphite in wine using a microbial sensor |
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Authors: | Kazuo Nakamura Yoshifumi Amano Ooki Nakayama |
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Affiliation: | (1) Department of Fermentation Technology, Faculty of Engineering, Yamanashi University, 400 Kofu, Japan;(2) Department of Environmental Engineering, Faculty of Engineering, Yamanashi University, 400 Kofu, Japan |
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Abstract: | Summary A microbial sensor of immobilized Thiobacillus thiooxidans S3 cells was assembled to determine free sulphite in wine. Sulphite oxidation activity of the immobilized cells was sufficiently high for use even after 3 months storage at 4° C. The sensitivity of this sensor was 116 nA·1·mg-1 for sulphur dioxide. The relationship between the current decrease and the sulphur dioxide concentration was linear up to 17 mg·1-1. The sampling rate achieved was 10 min per sample including washing time. This sensor method needed no pretreatment of wine samples, and wines diluted with 5 mM sulphuric acid solution could be directly introduced in the computer-aided analysis system. The pigments in red wine did not disturbed the analysis. |
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