Effect of Bis(guanylhydrazones) on Growth and Polyamine Uptake in Plant Cells |
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Authors: | F Antognoni S Agostani C Spinelli M Koskinen H Elo N Bagni |
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Institution: | (1) Dipartimento di Biologia evoluzionistica sperimentale, Università di Bologna, via Irnerio 42, 40126 Bologna, Italy, IT;(2) Department of Pharmacy, University of Helsinki, P.O. Box 56, FIN-00014 University of Helsinki, Helsinki, Finland, FI |
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Abstract: | In the present work the effect of several bis(guanylhydrazones) on the growth of Helianthus tuberosus tuber explants was studied. Different aliphatic congeners of glyoxal bis(guanylhydrazone) were tested. Most of the compounds
displayed an inhibitory effect on growth, and a correlation between the structure of the molecule and the inhibitory activity
was observed. Experiments carried out with glyoxal bis(guanylhydrazone) and its congeners methyl-, ethylmethyl-, and methylpropylglyoxal
bis(guanylhydrazones) show that as the total number of side chain carbon atoms in the molecule increases, the inhibitory potency
also increases. A depletion of spermidine levels was also found in the explants treated with ethylmethylglyoxal bis(guanylhydrazone),
which turned out to be one of the most potent growth inhibitors. The addition of spermidine caused a significant reversion
of the antiproliferative action of glyoxal bis(guanylhydrazone). The effect of these compounds on spermidine uptake in protoplasts
isolated from carrot phloem parenchyma was also investigated. Only a slight competition was found when antagonists were present
at concentrations 20 times higher than the polyamine, thus suggesting that bis(guanylhydrazones) do not share, at least at
low concentrations, the polyamine transport system in plant cells.
Received January 10, 1997; accepted January 22, 1999 |
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Keywords: | , Bis(guanylhydrazones)—,Carrot protoplasts—,Growth—,Helianthus tuberosus—,Polyamines—,Uptake |
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