Isolation and characterization of macaque dendritic cells from CD34(+) bone marrow progenitors. |
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| Authors: | L M Pinchuk G Grouard-Vogel D M Magaletti R T Doty R G Andrews E A Clark |
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| Institution: | Regional Primate Research Center, University of Washington Medical Center, Seattle, Washington 98195, USA. |
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| Abstract: | We have developed a method for isolating and characterizing pigtailed macaque dendritic cells (DCs) generated from CD34(+) bone marrow (BM) progenitors based on methods previously developed for isolating human DCs. Macaque DCs displayed a characteristic morphology and were potent stimulators of allogeneic T cell proliferation. They expressed a set of DC-associated markers, such as MHC class II, CD1a, CD4, CD11a, CD40, CD58, CD80, CD83, CD86, and CXCR4. Macaque DCs, as well as peripheral blood CD4(+) T cells, were highly susceptible to HIV-2 infection, as detected by DNA-PCR. The expression of HIV-2 in macaque DCs was downregulated by treatment with the beta-chemokine RANTES. Macaque DCs will be useful for defining the in vivo role of DCs in HIV pathogenesis and for optimizing and testing peptide-DC vaccines or tolerizing regimens. |
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