| HPLC法测定藜豆中左旋多巴的含量 |
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| 引用本文： | 黄海滨,苏健,谭叶憧. HPLC法测定藜豆中左旋多巴的含量[J]. 广西植物, 2004, 24(5): 460-462 |
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| 作者姓名： | 黄海滨 苏健 谭叶憧 |
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| 作者单位： | 1. 广西中医学院制药厂,广西,南宁,530001
2. 广西中医学院药学院,广西,南宁,530001 |
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| 摘 要： | 采用HPLC法建立测定藜豆中左旋多巴含量的方法。色谱柱为岛津C-18甲基硅烷柱(250 min×4.6mm,5μm),流动相为甲醇:0.1 mol／L醋酸溶液(25:75,v／v),以硫唑嘌呤为内标物,检测波长280 nm,流速1.0 mL／min,柱温为室温,供试品的前处理方法为微波辅助提取法。结果表明,该方法准确度高,线性关系好,重现性好,平均加样回收率为97.34％,RSD 1.01％(n=5),结果满意。
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| 关 键 词： | 藜豆 左旋多巴 高效液相色谱法 |
| 文章编号： | 1000-3142(2004)05-0460-03 |
| 修稿时间： | 2004-02-23 |
Determination of Levodopa in Mucuna macrocarpa Wall by HPLC |
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| HUANG Hai-bin,SU Jian,TAN Ye-chong. Determination of Levodopa in Mucuna macrocarpa Wall by HPLC[J]. Guihaia, 2004, 24(5): 460-462 |
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| Authors: | HUANG Hai-bin SU Jian TAN Ye-chong |
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| Abstract: | To establish a method for determination of Levodopa in Mucuna macrocarpa Wall by HPLC. Using HPLC with Shimadzn C-18 column(250 mm×4. 6 mm,5μm),the mixture of methanol and 0. 1 mol/L acetic acid(25 : 75,v/v)as mobile phase,detection wavelength is 280nm;Azathioprine was used as internal standard substance,flow rate at 1. 0 mL/min,column temperature at r. m. The method has a good linear dependence. The average recovery of loading sample was 97. 34% with RSD 1. 01% (n = 5). The method is fast,simple and stable. It has been proved to be a good practice for determination of Levodopa in M. macrocarpa Wall. |
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| Keywords: | Mucuna macrocarpa Wall Levodopa HPLC |
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