The remotor muscle of the lobster antenna: sarcoplasmic reticulum and skinned fiber experiments |
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| Authors: | M Villaz M Ronjat M Garrigos Y Dupont |
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| Affiliation: | 1. Laboratoire de Biophysique Moléculaire et Cellulaire (CNRS UA 520), Département de Recherche Fondamentale, Centre d''Etudes Nucléaires de Grenoble, F-38041-Grenoble, France;1. Service de Biophysique, Département de Biologie, Centre d''Etudes Nucléaires de Saclay, F-91191-Gif-sur-Yvette, France;1. Department of Entomology, University of Kentucky, Lexington, KY 40546, USA;2. Department of Horticulture, Oregon State University, Corvallis, OR 97330, USA;1. College of Life Sciences, Beijing Normal University, Beijing 100875, China;2. National Institute of Biological Sciences, No. 7 Science Park Road, Zhongguancun Life Science Park, Beijing 102206, China;3. Tsinghua Institute of Multidisciplinary Biomedical Research, Tsinghua University, Beijing 102206, China;1. Brain/Liver Interface Medicine Research Center, Kanazawa University, 13-1 Takaramachi, Kanazawa, Ishikawa 920-8640, Japan;2. Mathematical Neuroscience Unit, Institute for Frontier Science Initiative, Kanazawa University, 13-1 Takaramachi, Kanazawa, Ishikawa 920-8640, Japan;3. Laboratory of Developmental Neurobiology, Graduate School of Medical Sciences, Kanazawa University, 13-1 Takaramachi, Kanazawa, Ishikawa 920-8640, Japan;4. CREST, JST, 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan |
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| Abstract: | The striated remotor muscle of the lobster antenna has an extraordinarily profuse sarcoplasmic reticulum as shown by electron microscopy. Gel electrophoresis reveals a simple protein composition in which the Ca2+-ATPase predominates. Vesicles of sarcoplasmic reticulum (SR) from this remotor are shown to operate Ca2+ binding, Ca2+ transport, and Ca2+-activated hydrolysis of ATP with an usual efficiency (2 Ca2+ transported per ATP hydrolysed, 4 mumol ATP hydrolysed/mg protein/min). Skinned fiber experiments were performed. They indicate behaviour of the remotor expected from observations by EM and gel electrophoresis: contraction of low maximal intensity under Ca2+ excitation, long internal diffusion time due to the large volume of SR to be crossed, and large Ca2+ content released in a caffeine-sensitive manner. |
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