cDNA cloning and characterization of a new stress-responsive gene BoRS1 from Brassica oleracea var. acephala |
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| Authors: | Dongqin Tang Hongmei Qian Shunwu Yu Youfang Cao Zhihua Liao Lingxia Zhao Xiaofen Sun Danfeng Huang Kexuan Tang |
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| Institution: | Plant Biotechnology Research Center, Fudan-SJTU-Nottingham Plant Biotechnology R &D Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200030, China; Department of Landscape Architectural Science and Engineering, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 201101, China; State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan-SJTU-Nottingham Plant Biotechnology R &D Center, Morgan-Tan International Center for Life Sciences, Fudan University, Shanghai 200433, China |
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| Abstract: | A new stress-responsive gene BoRS1 (GenBank accession No. AY373021 ) was isolated from Brassica oleracea var. acephala by rapid amplification of cDNA ends (RACE). The full-length cDNA of BoRS1 was 2076 bp and contained a 1851 bp open reading frame (ORF) encoding 617 amino acids. Sequence analysis indicated that the deduced BoRS1 shared some identities with LTI65 , RD29A, RD29B and COR78 from Arabidopsis thaliana . Southern blot analysis of genomic DNA indicated that other related genes existed and there were two copies of BoRS1 in the genome of B. oleracea . Northern blot analysis revealed that BoRS1 was up-regulated by cold, mannitol, NaCl and abscisic acid (ABA). Expressional fluctuation of time course with ABA implied a two-step induction process. Tissue-specific expression analysis indicated that BoRS1 was expressed in all the tested plant tissues including leaves, stems and roots. Our studies imply that BoRS1 is a new gene that is responsive to environmental stresses such as low temperature, salinity and osmotic stress. |
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