Construction of a high-resolution genetic map and YAC-contigs in the tomato Tm-2a region |
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| Authors: | K. Pillen M. W. Ganal S. D. Tanksley |
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| Affiliation: | (1) Present address: Institute of Agronomy, University of Bonn, Katzenburgweg 5, 53115 Bonn, Germany;(2) Department of Plant Breeding and Biometry, 252 Emerson Hall, Cornell University, 14853 Ithaca, NY, USA;(3) Institute of Plant Genetics and Crop Plant Research, Corrensstr. 3, 06466 Gatersleben, Germany |
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| Abstract: | With the ultimate goal of cloning the Tobacco Mosaic Virus (TMV) resistance gene Tm-2a from tomato by means of positional cloning, a high-resolution map of a 4.3-cM region surrounding the Tm-2a gene has been constructed. In total, 13 RFLP and RAPD markers were mapped in close proximity to Tm-2a using 2112 individuals from an intraspecific Lycopersicon peruvianum backcross. The closest flanking markers were separated from Tm-2a by 0.05 cM on each side. Only one marker, the cDNA clone R12, co-segregated with Tm-2a. In order to physically cover the Tm-2a region, R12 and the flanking DNA marker TG207 were used to select homologous YAC clones. To-date, two YAC-contigs spanning approximately 340 kb and 360 kb have been constructed. The data obtained from these experiments indicate that recombination around the centromere of chromosome 9 is extremely suppressed. |
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| Keywords: | Tomato TMV resistance RFLP High resolution map YAC contig |
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