A novel TIMP-insensitive type IV collagen-degrading metalloproteinase from murine metastatic sarcoma cells |
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Authors: | Y Kato K Ogawa S Yamamoto S Abe J Kishi T Hayakawa |
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Institution: | Bio-Science Laboratory, Asahi Chemical Industry Co. Ltd., Shizuoka, Japan. |
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Abstract: | A novel type IV collagen-degrading metalloproteinase was purified from the conditioned media of a murine metastatic sarcoma cell line. The molecular weight of the purified enzyme was determined to be 100 kDa by SDS-PAGE, while 700 kDa by gel filtration suggesting that the enzyme has a multimer structure. This enzyme degrades type IV collagen, but neither type I collagen nor casein. The failure of trypsin treatment to enhance the enzyme activity suggested that the purified enzyme did not require activation. Although the enzyme seems to be classified as a matrix metalloproteinase, it was inhibited by neither tissue inhibitor of metalloproteinases (TIMP) nor TIMP-2 and thus represents a novel type IV collagen-degrading metalloproteinase. |
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