Peroxidase production by carbon and nitrogen sources fed-batch culture of Arthromyces ramosus |
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Authors: | Hideo Tsujimura Masamitsu Takaya Kenji Katano Nobuya Matsumoto Yong Soo Park Mitsuyasu Okabe |
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Affiliation: | (1) United Graduate School of Agricultural Science, Gifu University, 1-1, Yanagido, 501-11 Gifu, Japan;(2) Bioprocess Development Center, Consumer Health Products Department, Suntory Ltd., 1023-1, Yamazaki, Shimamoto-cho, Mishima-gun, 618 Osaka, Japan;(3) Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, Ohya 836, 422 Shizuoka, Japan |
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Abstract: | Summary Carbon and nitrogen sources were investigated for improving peroxidase production by Arthromyces ramosus, a hyperproducer of peroxidase. Glucose as carbon source and a mixture of yeast extract and polypeptone at the ratio of 3 to 5 as nitrogen source in a production medium were shown to give the highest peroxidase activity. During the culture amino acids such as alanine, arginine, methionine, leucine, tyrosine and tryptophan were depleted. Therefore, glucose supplemented nitrogen source fed-batch culture was carried out and a peroxidase activity of 73 U/ml was obtained. This activity was 1.7 times higher than that of glucose fed-batch culture. This indicates that an adequate nitrogen source supply during the culture is effective for improving the peroxidase production by A. ramosus. |
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