Characterization of Methanosarcina mazei N2M9705 Isolated from an Aquaculture Fishpond |
| |
Authors: | Mei-Chin Lai Chi-Ming Shu Ming-Shing Chiou Tong-Yung Hong Ming-Jen Chuang Jack Jay Hua |
| |
Institution: | (1) Department of Botany, National Chung Hsing University, Taichung, 40227 Taiwan, R.O.C. , TW;(2) Food Research & Development Institute, Hsinchu, Taiwan, R.O.C. , TW |
| |
Abstract: | A new methanogenic isolate, designated as strain N2M9705 (=OCM 668), was isolated from an aquaculture fishpond near Wang-gong,
Taiwan. This strain grew on trimethylamine and methanol, but it did not catabolize H2-CO2, acetate, or formate. The cells were stained Gram-negative, nonmotile, irregular coccus 0.6–0.8 μm in diameter. Gas vacuoles
were observed and cell aggregated to form various sizes of granules. Cells grew optimally at 32°–37°C with 1% NaCl. The pH
range of growth was 6.2–7.4, and higher pH inhibited the cell growth. The cells grew well in minimal medium, but growth was
greatly stimulated by yeast extract and peptone. A comparison of 16S rDNA sequences of this organism phylogenetically related
to Methanosarcina mazei. This is the first report of methyltrophic methanogenic isolated from an aquaculture fishpond.
Received: 16 March 1999 / Accepted: 16 April 1999 |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|