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Uridine phosphorylase activity among the class mollicutes
Authors:Dr. Gerard J. McGarrity  Lindsay Gamon  Theodor Steiner  Joseph Tully  Hitoshi Kotani
Affiliation:(1) Institute for Medical Research, Copewood Street, 08103 Camden, New Jersey, USA;(2) Mycoplasma Section, Laboratory of Molecular Microbiology, NIAID, Frederick, Maryland, USA
Abstract:Uridine phosphorylase activity has been used to detect mycoplasmas in cell cultures by measuring formation of14C-uracil from14C-uridine. In this report we show that all species ofMycoplasma, Acholeplasma, andUreaplasma tested exhibited uridine phorphorylase activity. Among the genusSpiroplasma, serogroups I-1, I-3, I-5, I-7, I-8, IV, XIII, and XIV lacked uridine phosphorylase activity.Present address: Ciba-Geigy, Basel, Switzerland.
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