Uridine phosphorylase activity among the class mollicutes |
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Authors: | Dr. Gerard J. McGarrity Lindsay Gamon Theodor Steiner Joseph Tully Hitoshi Kotani |
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Affiliation: | (1) Institute for Medical Research, Copewood Street, 08103 Camden, New Jersey, USA;(2) Mycoplasma Section, Laboratory of Molecular Microbiology, NIAID, Frederick, Maryland, USA |
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Abstract: | Uridine phosphorylase activity has been used to detect mycoplasmas in cell cultures by measuring formation of14C-uracil from14C-uridine. In this report we show that all species ofMycoplasma, Acholeplasma, andUreaplasma tested exhibited uridine phorphorylase activity. Among the genusSpiroplasma, serogroups I-1, I-3, I-5, I-7, I-8, IV, XIII, and XIV lacked uridine phosphorylase activity.Present address: Ciba-Geigy, Basel, Switzerland. |
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