Screening and preliminary characterization of hemagglutinins in Vietnamese marine algae |
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Authors: | Hung Le Dinh Kanj Hori Nang Huynh Quang |
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Institution: | (1) Graduate School of Biosphere Science, Hiroshima University, Kagamiyama 1–4–4, Higashi–Hiroshima 739-8528, Japan;(2) Institute of Materials Science Nhatrang Branch (VAST), 2A Hungvuong Street, Nhatrang, Vietnam |
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Abstract: | Extracts from 44 species of Vietnamese marine algae, including 15 Chlorophyta, 18 Rhodophyta and 11 Phaeophyta species, were
examined for hemagglutination activity with a variety of different animal and human erythrocytes that were untreated or treated
with enzymes. Almost all extracts showed activity toward at least one type of erythrocytes, although those from three Chlorophyta
and two Rhodophyta species showed no hemagglutination with any type of erythrocytes examined. Strong activity was detected
in extracts from two Chlorophyta (Anadyomene plicata and Avrainvillea erecta) and four Rhodophyta species (Gracilaria eucheumatoides, Gracilaria salicornia, Kappaphycus alvarezii, and Kappaphycus striatum) with enzyme-treated rabbit and sheep erythrocytes. The hemagglutinins of seven Chlorophyta and eight Rhodophyta species
were examined for sugar-binding specificity, pH- and temperature-stability, and divalent cation-independency of hemagglutination
using ammonium sulfate-precipitates prepared from their extracts. In a hemagglutination-inhibition test with various monosaccharides
and glycoproteins, none of the hemagglutinins had affinity for monosaccharides, except the Codium arabicum and Gracilaria euchematoides hemagglutinins, whose activities were inhibited by both N-acetyl-d-galactosamine and N-acetyl-d-glucosamine. On the other hand, all of the hemagglutinins activities were inhibited by some glycoproteins. The inhibition
profiles with glycoproteins were different depending on hemagglutinin species, and suggest the presence of lectins specific
for high mannose N-glycans, complex N-glycans, or O-glycans. The activities of these algal hemagglutinins were stable over
a wide range of pH and temperature, and independent of the presence of divalent cations. These results indicate that Vietnamese
marine algae are a good source of novel and useful lectins. |
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Keywords: | Carbohydrate-binding specificity Hemagglutinins Lectins Stability Survey Vietnamese marine algae |
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