Purification of acid ethanol-extracted human lymphoid interferons by Blue Sepharose chromatography. |
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| Authors: | J S Erickson K Paucker |
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| Affiliation: | Department of Microbiology, The Medical College of Pennsylvania, 3300 Henry Avenue, Philadelphia, Pennsylvania 19129 USA |
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| Abstract: | Human leukocyte and lymphoblastoid (Namalva) interferons were purified by a modified acid ethanol extraction procedure and chromatography of the dilute ethanolic interferon solution on Blue Sepharose. Both interferons were purified more than 1000-fold, with recoveries ranging from 35 to 40%. |
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