Temperature-dependent osmotic permeability in glycoproteion containing liposomes |
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Authors: | Vassil Z Neitchev François A Bideaud |
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Affiliation: | (1) Central Laboratory of Biophysics, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria;(2) 24 rue du Fauborg Saint-Jacques, 75014 Paris, France |
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Abstract: | The osmotic water outflow of large multilamellar liposomes containing 1-acid glycoprotein was measured at a temperature near the lipid's phase transition temperature. The liposomes were formed from a mixture of DPPC, cholesterol and glycoprotein in molar ratios 100:20:1, by continuous sucrose density gradient centrifugation. These liposomes captured 35% of the radiolabeled glycoprotein. The temperature-dependent experiments showed that near phase transition temperature the initial rate of water outflow increased drastically in comparison with glycoprotein free liposomes incubated in buffer containing glycoprotein. We suggested that eventual a channel mechanism may be involved due to spontaneous incorporation of glycoprotein into the bilayer. |
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Keywords: | lipid phase transition osmotic permeability liposomes glycoprotein |
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