The effect of thawing velocity on survival and acrosomal integrity of ram spermatozoa frozen at optimal and suboptimal rates in straws |
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| Authors: | P S Fiser R W Fairfull G J Marcus |
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| Institution: | 1. Clinic for Cattle, University of Veterinary Medicine Hannover, Bischofsholer Damm 15, 30173, Germany;2. ETH Zürich, Animal Physiology, Institute of Agricultural Sciences, Department of Environmental Systems Science, Universitätsstrasse 2, 8092, Zurich, Switzerland;3. Vetsuisse Faculty, Department of Clinical Veterinary Science, Clinic for Ruminants, University of Bern, Hochschulstrasse 6, 3012, Bern, Switzerland;4. Cantonal Veterinary Service of Berne, Münsterplatz 3a, 3011, Bern, Switzerland;5. Institute for Anatomy, University of Veterinary Medicine Hannover, Bischofsholer Damm 15, 30173, Germany;6. Clinic for Animal Reproduction Medicine, University of Zurich, Winterthurerstrasse 260, CH-8057, Zurich, Switzerland;1. Department of Veterinary Medicine, College of Agriculture and Veterinary Medicine, Qassim University, Qassim, Saudi Arabia;2. Department of Animal Production, College of Agriculture and Veterinary Medicine, Qassim University, Qassim, Saudi Arabia;3. Department of Theriogenology, Faculty of Veterinary Medicine, Assiut University, Assiut, Egypt;1. Department of Theriogenology, Faculty of Veterinary Medicine, Kafrelsheikh University, El-Geish Street, 33516 Kafrelsheikh, Egypt;2. Riwina Animal Production Station, Agriculture Production Sector, Agricultural Research Center, Ministry of Agriculture, Kafrelsheikh, Egypt;1. School of Source and Environmental Science, Wuhan University, Wuhan, 430072, China;2. School of Power and Mechanical Engineering, Wuhan University, Wuhan 430072, China |
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| Abstract: | The effect of various thawing velocities on the motility and acrosomal maintenance of ram spermatozoa frozen at 20 degrees C/min (optimal) or 2 degrees C/min (suboptimal) was studied. The freeze-thaw motility and the percentage of intact acrosomes of spermatozoa frozen at 20 degrees C/min increased progressively with the thawing velocity. In semen frozen at 2 degrees C/min, motility of spermatozoa and the percentage of intact acrosomes declined drastically when the thawing velocity obtained in air at 20 degrees C was increased by thawing in water at 20 degrees C. Thawing at higher temperatures markedly increased both motility and acrosomal preservation, but the best results with semen frozen at 2 degrees C/min were lower than those obtained with semen frozen at 20 degrees C/min. The optimal freeze-thaw conditions for semen protected by 4% glycerol were freezing at 20 degrees C/min and thawing in water at 60 or 80 degrees C for 8 or 5 sec, respectively. Semen collected from rams exposed to a decreasing photoperiod exhibited higher motility after freezing and thawing than those exposed to an increasing photoperiod. However, there was no effect on acrosomal preservation after freezing at 20 degrees C/min. |
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